confocal laser scanning microscopy (Olympus)
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confocal laser scanning microscopy
Confocal Laser Scanning Microscopy, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 24448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fv3000+laser+scanning+confocal+microscopy/FV3000+Confocal+Laser+Scanning+Microscope/pm41928263-128-13-18
Average 99 stars, based on 24448 article reviews
Confocal Laser Scanning Microscopy, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 24448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fv3000+laser+scanning+confocal+microscopy/FV3000+Confocal+Laser+Scanning+Microscope/pm41928263-128-13-18
Average 99 stars, based on 24448 article reviews
confocal laser scanning microscopy - by Bioz Stars,
2026-09
99/100 stars
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Confocal Microscopy:Article Title: Mitochondrial RelA empowers mtDNA G-quadruplex formation for hypoxia adaptation in cancer cells. Article Snippet: Article Mitochondrial RelA empowers mtDNA G-quadruplex formation for hypoxia adaptation in cancer cells Article Title: Integration of heart-on-a-chip and metabolomics for understanding the toxicity-attenuating effect of ethnomedicinal processing. Article Snippet: Background: Tiebangchui (TBC), is a well-known traditional Tibetan medicine that coexists with toxicity and effects.. Highland barley wine is an effective and unique processing method to reduce TBC’s toxic side effects.. However, the toxicity reduction mechanism is ambiguous and needs to be explored urgently. Article Title: The ER protein CANX (calnexin)-mediated autophagy protects against alzheimer disease. Article Snippet: Although the relationship between macroautophagy/autophagy and Alzheimer disease (AD) is widely studied, the underlying mechanisms are poorly understood, especially the regulatory role of the initiation signaling of autophagy on AD.. Here, we find that the ER transmembrane protein CANX (calnexin) is a novel interaction partner of the autophagy-inducing kinase ULK1 and is required for ULK1 recruitment to the ER under basal or starved conditions.. Loss of CANX results in the inactivity of ULK1 kinase and inhibits autophagy flux. Article Title: A novel MTORC2-AKT-ROS axis triggers mitofission and mitophagy-associated execution of colorectal cancer cells upon drug-induced activation of mutant KRAS. Article Snippet: RAS is one of the most commonly mutated oncogenes associated with multiple cancer hallmarks.. Notably, RAS activation induces intracellular reactive oxygen species (ROS) generation, which we previously demonstrated as a trigger for autophagy-associated execution of mutant KRASexpressing cancer cells.. Here we report that drug (merodantoin; C1)-induced activation of mutant KRAS promotes phospho-AKT S473-dependent ROS-mediated S616 phosphorylation and mitochondrial localization of DNM1L/DRP1 (dynamin 1 like) and cleavage of the fusion-associated protein OPA1 (OPA1 mitochondrial dynamin like GTPase). Article Title: Dihydrotanshinone I inhibits gallbladder cancer growth by targeting the Keap1-Nrf2 signaling pathway and Nrf2 phosphorylation. Article Snippet: .. Finally, the cells were examined using an Olympus Article Title: Development of a high-throughput sensitive SB-PCR assay for rapid detection of laboratory-escaped antibiotic resistance genes Article Snippet: Laboratory wastewater contains engineered bacteria with antibiotic resistance genes (ARGs) and antibiotics, creating a multidrug stress that promotes multidrug-resistant strains and accelerates ARGs spread.. Therefore, dynamic monitoring of ARGs in such wastewater is essential.. This study presents a novel solid-liquid biphasic PCR (SB-PCR) platform based on suspension array technology for rapid detection of laboratory-originated ARGs. Software:Article Title: Integration of heart-on-a-chip and metabolomics for understanding the toxicity-attenuating effect of ethnomedicinal processing. Article Snippet: Background: Tiebangchui (TBC), is a well-known traditional Tibetan medicine that coexists with toxicity and effects.. Highland barley wine is an effective and unique processing method to reduce TBC’s toxic side effects.. However, the toxicity reduction mechanism is ambiguous and needs to be explored urgently. Fluorescence:Article Title: The ER protein CANX (calnexin)-mediated autophagy protects against alzheimer disease. Article Snippet: Although the relationship between macroautophagy/autophagy and Alzheimer disease (AD) is widely studied, the underlying mechanisms are poorly understood, especially the regulatory role of the initiation signaling of autophagy on AD.. Here, we find that the ER transmembrane protein CANX (calnexin) is a novel interaction partner of the autophagy-inducing kinase ULK1 and is required for ULK1 recruitment to the ER under basal or starved conditions.. Loss of CANX results in the inactivity of ULK1 kinase and inhibits autophagy flux. Plasmid Preparation:Article Title: A novel MTORC2-AKT-ROS axis triggers mitofission and mitophagy-associated execution of colorectal cancer cells upon drug-induced activation of mutant KRAS. Article Snippet: RAS is one of the most commonly mutated oncogenes associated with multiple cancer hallmarks.. Notably, RAS activation induces intracellular reactive oxygen species (ROS) generation, which we previously demonstrated as a trigger for autophagy-associated execution of mutant KRASexpressing cancer cells.. Here we report that drug (merodantoin; C1)-induced activation of mutant KRAS promotes phospho-AKT S473-dependent ROS-mediated S616 phosphorylation and mitochondrial localization of DNM1L/DRP1 (dynamin 1 like) and cleavage of the fusion-associated protein OPA1 (OPA1 mitochondrial dynamin like GTPase). Article Title: A novel MTORC2-AKT-ROS axis triggers mitofission and mitophagy-associated execution of colorectal cancer cells upon drug-induced activation of mutant KRAS Article Snippet: About 50,000 HCT116 cells were seeded on ibidi black multi-well 24-well plates (ibidi GmbH, Gräfelfing, Germany) and transfection of pEGFP-C1-LC3 plasmid was carried out the day after via jetPRIME® transfection reagent. .. Briefly 50 μL of jetPRIME® buffer with 0.5 μg of pEGFP-C1-LC3 plasmid and 1 μL of jetPRIME® transfection reagent was added into the cells and left for 24 h. EGFP-LC3 overexpression was confirmed via Olympus Transfection:Article Title: A novel MTORC2-AKT-ROS axis triggers mitofission and mitophagy-associated execution of colorectal cancer cells upon drug-induced activation of mutant KRAS. Article Snippet: RAS is one of the most commonly mutated oncogenes associated with multiple cancer hallmarks.. Notably, RAS activation induces intracellular reactive oxygen species (ROS) generation, which we previously demonstrated as a trigger for autophagy-associated execution of mutant KRASexpressing cancer cells.. Here we report that drug (merodantoin; C1)-induced activation of mutant KRAS promotes phospho-AKT S473-dependent ROS-mediated S616 phosphorylation and mitochondrial localization of DNM1L/DRP1 (dynamin 1 like) and cleavage of the fusion-associated protein OPA1 (OPA1 mitochondrial dynamin like GTPase). Article Title: A novel MTORC2-AKT-ROS axis triggers mitofission and mitophagy-associated execution of colorectal cancer cells upon drug-induced activation of mutant KRAS Article Snippet: About 50,000 HCT116 cells were seeded on ibidi black multi-well 24-well plates (ibidi GmbH, Gräfelfing, Germany) and transfection of pEGFP-C1-LC3 plasmid was carried out the day after via jetPRIME® transfection reagent. .. Briefly 50 μL of jetPRIME® buffer with 0.5 μg of pEGFP-C1-LC3 plasmid and 1 μL of jetPRIME® transfection reagent was added into the cells and left for 24 h. EGFP-LC3 overexpression was confirmed via Olympus Over Expression:Article Title: A novel MTORC2-AKT-ROS axis triggers mitofission and mitophagy-associated execution of colorectal cancer cells upon drug-induced activation of mutant KRAS. Article Snippet: RAS is one of the most commonly mutated oncogenes associated with multiple cancer hallmarks.. Notably, RAS activation induces intracellular reactive oxygen species (ROS) generation, which we previously demonstrated as a trigger for autophagy-associated execution of mutant KRASexpressing cancer cells.. Here we report that drug (merodantoin; C1)-induced activation of mutant KRAS promotes phospho-AKT S473-dependent ROS-mediated S616 phosphorylation and mitochondrial localization of DNM1L/DRP1 (dynamin 1 like) and cleavage of the fusion-associated protein OPA1 (OPA1 mitochondrial dynamin like GTPase). Article Title: A novel MTORC2-AKT-ROS axis triggers mitofission and mitophagy-associated execution of colorectal cancer cells upon drug-induced activation of mutant KRAS Article Snippet: About 50,000 HCT116 cells were seeded on ibidi black multi-well 24-well plates (ibidi GmbH, Gräfelfing, Germany) and transfection of pEGFP-C1-LC3 plasmid was carried out the day after via jetPRIME® transfection reagent. .. Briefly 50 μL of jetPRIME® buffer with 0.5 μg of pEGFP-C1-LC3 plasmid and 1 μL of jetPRIME® transfection reagent was added into the cells and left for 24 h. EGFP-LC3 overexpression was confirmed via Olympus Amplification:Article Title: Development of a high-throughput sensitive SB-PCR assay for rapid detection of laboratory-escaped antibiotic resistance genes Article Snippet: Laboratory wastewater contains engineered bacteria with antibiotic resistance genes (ARGs) and antibiotics, creating a multidrug stress that promotes multidrug-resistant strains and accelerates ARGs spread.. Therefore, dynamic monitoring of ARGs in such wastewater is essential.. This study presents a novel solid-liquid biphasic PCR (SB-PCR) platform based on suspension array technology for rapid detection of laboratory-originated ARGs. Double Staining:Article Title: Integration of heart-on-a-chip and metabolomics for understanding the toxicity-attenuating effect of ethnomedicinal processing. Article Snippet: Background: Tiebangchui (TBC), is a well-known traditional Tibetan medicine that coexists with toxicity and effects.. Highland barley wine is an effective and unique processing method to reduce TBC’s toxic side effects.. However, the toxicity reduction mechanism is ambiguous and needs to be explored urgently. |